Research

My research focuses on bioanalytical method development and validation using liquid chromatography-tandem mass spectrometry (LC-MS/MS).  Many of the lab's current projects focus quantification of endogenous compounds, such as acetylcholine, estradiol, and progesterone and compounded drug stability.  Our collaborators include Johnson City Medical Center, Azurity Pharmaceuticals, Inc., the ETSU Quillen College of Medicine, among others.  

Featured Students

Sarah Beth Lawson, P4, has worked on a collaboration with Dr. Donald Hoover's lab since 2018.  She is presenting her work at the 2019 ASHP-Midyear Clinical Meeting in Las Vegas, NV.

Tim Archibald, P4, received 1st place in his division at the 2019  Appalachian Student Research Forum. His work was recently published in the Journal of Pharmacy Technology.

Featured Project:
Stability of ampicillin in normal saline following refrigerated storage and 24-hour pump re-circulation
Authors: Mariah Huskey, BS, PharmD Candidate, Paul Lewis, PharmD, BCPD (AQ-ID), Stacy Brown, PhD

T. Archibald and S.D. Brown. “Monitoring Commercials Ibuprofen Potency Changes Over 1 Year When Stored in a Household Setting.” Journal of Pharmacy Technology. https://doi.org/10.1177/8755122519877808, 2019.

S. Lawson, P.O. Lewis, G. Peacock, and S.D. Brown. “Comparative Stability of Oral Vitamin K Liquids Stored in Refrigerated Amber Plastic Syringes.” Journal of Pharmacy Technology. 35(3): 105-109, 2019.

A. Gonzalez-Estrada, T. Archibald, B. Campbell, K. Dinsmore, G. Mosier, S. Brown. “Stability of Diluted Neuromuscular Blocking Agents Utilized in Perioperative Hypersensitivity Evaluation.”  Allergy, European Journal of Allergy and Clinical Immunology.  73(12): 2398-2400, 2018.

J. Huffman, S. Brown, P. Lewis, S. Lawson, A. Ogle, G. Peacock, “Comparative Stability of Vitamin K Oral Liquids in Sterile Water for Injection and Stored in Amber Glass Bottles and Amber Plastic Syringes.” International Journal of Pharmaceutical Compounding.  22(4): 340-344, 2018.

Study Objective

Use of ampicillin in outpatient parenteral antimicrobial therapy (OPAT) has historically been complicated by frequent dosing and short beyond use dates. This study investigates the possibility of preparing ampicillin in normal saline and storing it prior to use.

Study Design

  • 2-g vials ampicillin reconstituted in 10-mL SWFI and injected into a 250-mL IV bag of SWFI and initial concentrations determined using stability-indicating HPLC-UV assay

  • Preparations were set up to circulate through an infusion pump for 24 hrs following refrigerated storage (4 batches per experimental group): Immediate use, 24-hr storage, 72-hr storage, 7-d storage

  • Samples (1-mL aliquots) were pulled from re-circulation at 4, 8, 12, and 24 hours and assayed for ampicillin concentration

Conclusion

Ampicillin can be prepared in SWFI and stored in a refrigerator for up to 72-hours prior to continuously infusing at room temperature over 24-hours with less than a 10% loss of potency.